Various lysates were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANKRD1 antibody (67775-1-Ig, Clone: 1F11F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).