1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were then stained with 5 ul CoraLite® Plus 488 Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8a (RPA-T8) (65144-1-Ig) or 0.2 ug Mouse IgG1 Isotype Control and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD8a (RPA-T8) (APC-65144, Clone:RPA-T8), and 5 ul APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21). Cells were not fixed.
1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug PE-anti-human CD8a (PE-65144, clone RPA-T8) (red) or 0.125 ug PE-mouse IgG1 isotype control (blue). Cells were not fixed.
1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD8a (CL488-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD8a (CL488-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with CL488 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD8a (CL647-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD8a (CL647-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD8a (CL488-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul FITC Anti-Human CD8a (FITC-65144, Clone:RPA-T8), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.