CD11c/Integrin Alpha X Monoclonal antibody
CD11c/Integrin Alpha X Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2F1C10
Cat no : 60258-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human spleen tissue, THP-1 cells, HL-60 cells, U-937 cells |
| Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human liver cancer tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human appendicitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 5 publications below |
| IF | See 17 publications below |
Product Information
60258-1-Ig targets CD11c/Integrin Alpha X in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD11c/Integrin Alpha X fusion protein Ag11350 |
| Full Name | integrin, alpha X (complement component 3 receptor 4 subunit) |
| Calculated Molecular Weight | 1169 aa, 129 kDa |
| Observed Molecular Weight | 145 kDa |
| GenBank Accession Number | BC038237 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 3687 |
| RRID | AB_2881379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig | Download protocol |
| IHC protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig | Download protocol |
| IF protocol for CD11c/Integrin Alpha X antibody 60258-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer. | ||
Sci Immunol Initiator cell death event induced by SARS-CoV-2 in the human airway epithelium | ||
J Exp Med AIM2 regulates anti-tumor immunity and is a viable therapeutic target for melanoma. | ||
Diabetes Loss of IDO1 Expression From Human Pancreatic β-Cells Precedes Their Destruction During the Development of Type 1 Diabetes. | ||
J Invest Dermatol Lidocaine Ameliorates Psoriasis by Obstructing Pathogenic CGRP Signaling-Mediated Sensory Neuron-Dendritic Cell Communication. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue at 1:500. We used a Tris-EDTA pH 9.0 antigen retrieval.
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