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  • Featured Product
  • KD/KO Validated

CD44 Monoclonal antibody

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

6F4H2

Cat no : 60224-1-Ig

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD44 Monoclonal antibody (60224-1-Ig)

    WB analysis using 60224-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using CD44 Monoclonal antibody (60224-1-Ig)

    WB analysis using 60224-1-Ig

    HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CD44 Monoclonal antibody (60224-1-Ig)

    WB analysis of HeLa cells using 60224-1-Ig

    WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells.

  • Western Blot (WB) analysis of human spleen tissue using CD44 Monoclonal antibody (60224-1-Ig)

    WB analysis of human spleen using 60224-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CD44 Monoclonal antibody (60224-1-Ig)

    IP experiment of HeLa using 60224-1-Ig

    IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human lung cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human lung cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human oesophagus cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human oesophagus cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human breast cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human breast cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human lung cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human tonsillitis using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human tonsillitis using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IHC staining of human lung cancer using 60224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IF Staining of human lung cancer using 60224-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CD44 Monoclonal antibody (60224-1-Ig)

    IF Staining of human lung cancer using 60224-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD44 Monoclonal antibody (60224-1-Ig)

    IF Staining of A431 using 60224-1-Ig

    Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD44 Monoclonal antibody (60224-1-Ig)

    IF Staining of A431 using 60224-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Tested Applications

Positive WB detected inMDA-MB-231 cells, human spleen tissue, HeLa cells, A549 cells, U-251 cells, HUVEC cells, pig spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60224-1-Ig targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD44 fusion protein Ag7633
Full Name CD44 molecule (Indian blood group)
Calculated Molecular Weight 742 aa, 82 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
RRIDAB_11042767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Protocols

Product Specific Protocols
WB protocol for CD44 antibody 60224-1-IgDownload protocol
IHC protocol for CD44 antibody 60224-1-IgDownload protocol
IF protocol for CD44 antibody 60224-1-IgDownload protocol
IP protocol for CD44 antibody 60224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nature

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Authors - Lei Han
View Article
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
View Article
humanIF

Biomater Sci

Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels

Authors - Xiaozhang Zhang
View Article
humanIF

Biomater Sci

Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents

Authors - Conghui Tian
View Article
ratIF

Stem Cell Res Ther

Mesenchymal stem cells in synovial fluid increase in number in response to synovitis and display more tissue-reparative phenotypes in osteoarthritis

Authors - Hideto Furuoka
View Article
mouseWB

J Nanobiotechnology

Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.

Authors - Hongying Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma cells