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  • Featured Product
  • KD/KO Validated

Neprilysin/CD10 Monoclonal antibody

Neprilysin/CD10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-Ig

Synonyms

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis using 60034-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis of Daudi using 60034-3-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis using 60034-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis of HEK-293 using 60034-3-Ig

    WB result of Neprilysin/CD10 antibody (60034-3-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neprilysin/CD10 transfected HEK-293 cells.

  • Western Blot (WB) analysis of Ramos cells using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis of Ramos using 60034-3-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis of pig kidney using 60034-3-Ig

    pig kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    WB analysis of mouse kidney using 60034-3-Ig

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human stomach cancer using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human stomach cancer using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human renal cell carcinoma using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human renal cell carcinoma using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:100000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60034-3-Ig (Neprilysin/CD10 antibody) at dilution of 1:50000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsil tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human tonsil using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsil tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of human tonsil using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsil using 60034-3-Ig(Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of mouse kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of mouse kidney using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of mouse liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Neprilysin/CD10 Monoclonal antibody (60034-3-Ig)

    IHC staining of mouse liver using 60034-3-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60034-3-Ig (Neprilysin/CD10 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, Daudi cells, HEK-293 cells, mouse kidney tissue, pig kidney tissue, Ramos cells, A172 cells, rabbit kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human renal cell carcinoma tissue, human stomach cancer tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:25000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60034-3-Ig targets Neprilysin/CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Reactivity human, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neprilysin/CD10 fusion protein Ag0427
Full Name membrane metallo-endopeptidase
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC101658
Gene Symbol CD10
Gene ID (NCBI) 4311
RRIDAB_10641991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Protocols

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
IHC protocol for Neprilysin/CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols