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  • KD/KO Validated

C9orf72 Polyclonal antibody

C9orf72 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22637-1-AP

Synonyms

C9orf72, C9RANT, RP11 27J8.2



Tested Applications

Positive WB detected inC2C12 cells, transfected cells, HeLa cells, Sp2/0 cells, SH-SY5Y cells, rat brain tissue, mouse testis tissue, HEK-293 cells, NIH/3T3 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22637-1-AP is C9orf72 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Caenorhabditis elegans
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf72 fusion protein Ag18326
Full Name chromosome 9 open reading frame 72
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_10953528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)

Protocols

Product Specific Protocols
WB protocol for C9orf72 antibody 22637-1-APDownload protocol
IHC protocol for C9orf72 antibody 22637-1-APDownload protocol
IF protocol for C9orf72 antibody 22637-1-APDownload protocol
IP protocol for C9orf72 antibody 22637-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

C9orf72 is required for proper macrophage and microglial function in mice.

Authors - J G O'Rourke
  • KO Validated
humanwb

Nat Med

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Authors - Yingxiao Shi
human,mouseWB,CoIP

Cell Metab

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Authors - Tao Wang
  • KO Validated
human,mouseWB

Nat Neurosci

Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.

Authors - Qiang Zhu
  • KO Validated
humanWB

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
  • KO Validated
mouseWB

Sci Transl Med

Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

Authors - Aaron Burberry
  • KO Validated