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  • Featured Product
  • KD/KO Validated

P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18420-1-AP

Synonyms

P62/SQSTM1, P62, SQSTM1, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    WB analysis using 18420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    WB analysis of U2OS using 18420-1-AP

    U2OS (WT and SQSTM1 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 18420-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Western Blot (WB) analysis of various lysates using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    WB analysis using 18420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    WB analysis of HEK-293 using 18420-1-AP

    WB result of P62,SQSTM1 antibody (18420-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of U2OS cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IP experiment of U2OS using 18420-1-AP

    U2OS lysates prepared and IP of SQSTM1 performed using 1.0 μg of 18420-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HepG2 cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IP experiment of HepG2 using 18420-1-AP

    IP result of anti-P62,SQSTM1 (IP:18420-1-AP, 4ug; Detection:18420-1-AP 1:6000) with HepG2 cells lysate 1360 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human gliomas using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 18420-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human liver cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human liver cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human lung cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human lung cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human lung cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human lung cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18420-1-AP (P62,SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IHC staining of human liver cancer using 18420-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18420-1-AP (P62/SQSTM1 antibody at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IF Staining of HepG2 using 18420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using P62,SQSTM1 antibody (18420-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IF Staining of HeLa using 18420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (18420-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    IF Staining of U2OS using 18420-1-AP

    U2OS WT cells (yellow outline) and SQSTM1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 18420-1-AP at 1:300. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Flow cytometry (FC) experiment of HEK-293 cells using P62,SQSTM1 Polyclonal antibody (18420-1-AP)

    FC experiment of HEK-293 using 18420-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (18420-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, HEK-293 cells, HepG2 cells, U-87 MG cells, Jurkat cells
Positive IP detected inHepG2 cells, U2OS cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inChloroquine treated HepG2 cells, U2OS cells, Chloroquine treated HeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18420-1-AP targets P62,SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rabbit, monkey, chicken, zebrafish, hamster, goat, goslings
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131
Full Name sequestosome 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_10694431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Background

 P62 (ubiquitin-binding protein P62), also known as Sequestosome-1, is a multifunctional adaptor protein most widely known for its role as an autophagosome cargo protein (PMID: 8551575). P62 via specific interactions with polyubiquitylated target proteins induces their selective autophagy (PMID: 17580304). It also plays an important role in the regulation of the NFkB signaling pathway, senescence, cell differentiation, apoptosis, and immune responses (PMID: 26404812).

 What is the molecular weight of P62?

 The observed molecular weight of the protein can vary from as low as 8 kDa (for the smallest isoforms) to 48 kDa.

 What is the subcellular localization of P62?

 P62 is mainly localized in the cytoplasm; however, upon autophagy induction, e.g., via starvation or selective inhibitor treatment, it localizes in vesicular structures - autophagosomes.

 What is the tissue specificity of P62?

 It is ubiquitously expressed in various tissues.

 What is the function of P62 in the regulation of cell death and autophagy?

 It is a selective autophagy receptor that forms a bridge between polyubiquitylated cargo (via its UBA domains) and an autophagy modifier such as LC3 (via LIR domains) (PMIDs: 16286508, 20168092, 24128730, 28404643, 22622177). The process of selective autophagy is tightly regulated at many levels, including the posttranslational modifications (PTMs) of various proteins in the cascade, P62 among others (PMID: 29233872). P62 is involved in the regulation of cell death induction in response to various stimuli, e.g., via activation of caspase-8 at the autophagosome membrane (PMID: 29480462). In addition, P62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression.

 What is P62's involvement in disease?

 Mutations in P62 have been associated with the following diseases: sporadic and familial Paget's disease of bone, neurodegenerative diseases, diabetes, and obesity (PMID: 29480462). A growing number of reports suggest the implication of P62 in the induction of multiple cellular oncogenic transformations. Indeed, increased levels of P62 have been linked to tumor formation, cancer promotion, and resistance to therapy (PMID: 29738493). Moreover, P62 is an unfavorable prognostic marker in liver cancer. 


Protocols

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IHC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IP protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells.

Authors - Xiangbo Meng
View Article
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
View Article
humanWB

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
View Article
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
View Article
humanWB

Protein Cell

ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover

Authors - Maomao Pu
View Article
WB

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

David (Verified Customer) (01-02-2024)

Used for WB in cell lysates. Prominent band at the predicted molecular weight, although some non-specific bands in the control at around 40kDa. However, the correct SQSTM1 band can be clearly visualised.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y lysate
FH

Alexandru (Verified Customer) (11-21-2023)

Great at detecting p62 in cell lysates.

  • Applications: Western Blot
  • Cell Tissue Type: HEK-293
FH

Wan (Verified Customer) (02-24-2022)

Perfect antibody, clear bands!

  • Applications: Western Blot
FH

X (Verified Customer) (01-18-2021)

It is a reliable Ab to detect p62 with mouse brain lysate, although there is other band detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
P62,SQSTM1 Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:18420-1-AP)
FH

X (Verified Customer) (01-18-2021)

It is a reliable Ab to detect p62 with mouse brain lysate in WB, although there is other bands seen.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
FH

Wei (Verified Customer) (01-30-2020)

Good, Clear band for mouse heart

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Joleen (Verified Customer) (06-16-2019)

Recognizes SQSTM1 prominently with some non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
P62,SQSTM1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:18420-1-AP)