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Beclin 1 Polyclonal antibody

Beclin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

917

Reviews (6)

Cat no : 11306-1-AP

Synonyms

BECN1, Beclin-1-C 37 kDa, Beclin-1-C 35 kDa, Beclin-1, Beclin1

Validation Data Gallery

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AllWBIPIHCIF-PIF-FroIF/ICC

WB analysis of HEK-293 using 11306-1-AP
WB result of Beclin 1 antibody (11306-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beclin 1 transfected HEK-293 cells.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11306-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 11306-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11306-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 11306-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11306-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11306-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of MDA-MB-453s using 11306-1-AP
IP result of anti-Beclin 1 (IP:11306-1-AP, 4ug; Detection:11306-1-AP 1:1000) with MDA-MB-453s cells lysate 2800 ug.
IHC staining of human breast cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human stomach using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human stomach using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human cervical cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11306-1-AP (Beclin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast hyperplasia using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast hyperplasia using 11306-1-AP
Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 11306-1-AP (Beclin 1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11306-1-AP (Beclin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of mouse heart using 11306-1-AP
Immunofluorescent analysis ofun-fixed frozen OCT-embedded mouse heart tissue using Beclin 1 antibody (11306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of NIH/3T3 using 11306-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Beclin 1 antibody (11306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11306-1-AP

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Tested Applications

Positive WB detected in	C6 cells, HEK-293 cells, Raji cells, L02 cells, PC-3 cells, A431 cells, HT-1080 cells, human placenta tissue, mouse brain tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, MDA-MB-453s cells, SH-SY5Y cells
Positive IP detected in	MDA-MB-453s cells
Positive IHC detected in	human breast cancer tissue, human breast hyperplasia tissue, human prostate hyperplasia tissue, human cervical cancer tissue, human colon cancer tissue, human stomach tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue
Positive IF-Fro detected in	mouse heart tissue
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 21 publications below
WB	See 836 publications below
IHC	See 103 publications below
IF	See 74 publications below
IP	See 9 publications below
ELISA	See 1 publications below
CoIP	See 8 publications below

Product Information

11306-1-AP targets Beclin 1 in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, hamster, goat, fish, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Beclin 1 fusion protein Ag1843
Full Name	beclin 1, autophagy related
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	52-60 kDa
GenBank Accession Number	BC010276
Gene Symbol	Beclin 1
Gene ID (NCBI)	8678
RRID	AB_2259061
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Protocols

Product Specific Protocols
WB protocol for Beclin 1 antibody 11306-1-AP	Download protocol
IHC protocol for Beclin 1 antibody 11306-1-AP	Download protocol
IF protocol for Beclin 1 antibody 11306-1-AP	Download protocol
IP protocol for Beclin 1 antibody 11306-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival Authors - Pauline Lascaux View Article
human	WB,IP	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
mouse	IF	ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia Authors - Shishi Shen View Article
human	WB,IHC	Cancer Commun (Lond) Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies Authors - Lu Tang View Article
human	WB	Mol Cancer PMID: 32727463 Authors - Cefan Zhou View Article
human	WB,IF	Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia Authors - Tiemei Li View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH James (Verified Customer) (11-30-2022)	This antibody works well in WB Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: Liver Beclin 1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:11306-1-AP)
FH Jonathan (Verified Customer) (04-08-2022)	Antibody worked well - gave a nice, specific and strong signal. Happy. Applications: Western Blot Primary Antibody Dilution: 1 in 1000 Cell Tissue Type: Vero cells (African Green Monkey)
FH Zhengnan (Verified Customer) (02-24-2022)	The antibody is very good, got correct bands and background is clear! Applications: Western Blot
FH David (Verified Customer) (03-30-2020)	Used for immunoblot, single band at expected molecular weight, good signal to noise ratio Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: SH-SY5Y
FH Kanika (Verified Customer) (09-18-2019)	Works well at the recommended dilution for western blotting.Immunofluorescence signal was variable/low even with same concentration in replicate experiments. Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Human platelets Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in Human platelets (Cat no:11306-1-AP)
FH Kishor (Verified Customer) (01-30-2019)	Working well for Western Blotting (1:5000) and IF (1:300) Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000 Cell Tissue Type: H69, HUCCT-1 Beclin 1 Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11306-1-AP)

Beclin 1 Polyclonal antibody

Beclin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis of human placenta using 11306-1-AP

WB analysis using 11306-1-AP

WB analysis of A431 using 11306-1-AP

WB analysis of HT-1080 using 11306-1-AP

WB analysis of L02 using 11306-1-AP

WB analysis of PC-3 using 11306-1-AP

IP experiment of MDA-MB-453s using 11306-1-AP

IHC staining of human breast cancer using 11306-1-AP

IHC staining of human breast cancer using 11306-1-AP

IHC staining of human liver cancer using 11306-1-AP

IHC staining of human liver cancer using 11306-1-AP

IHC staining of human liver cancer using 11306-1-AP

IHC staining of human prostate hyperplasia using 11306-1-AP

IHC staining of human stomach using 11306-1-AP

IHC staining of human stomach using 11306-1-AP

IHC staining of human cervical cancer using 11306-1-AP

IHC staining of human cervical cancer using 11306-1-AP

IHC staining of human liver cancer using 11306-1-AP

IHC staining of human colon cancer using 11306-1-AP

IHC staining of human colon cancer using 11306-1-AP

IHC staining of human breast hyperplasia using 11306-1-AP

IHC staining of human breast hyperplasia using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of mouse heart using 11306-1-AP

IF Staining of NIH/3T3 using 11306-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

PMID: 32727463

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Reviews

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