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PLCG1 Recombinant antibody, PBS Only (Detector)

PLCG1 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242529A11

Cat no : 84941-1-PBS

Synonyms

Phospholipase C II, Phospholipase C gamma 1, Phosphoinositide phospholipase C-gamma-1, EC:3.1.4.11, 242529A11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PLCG1 Recombinant antibody (84941-1-RR)

    IF Staining of Jurkat using 84941-1-RR (same clone as 84941-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PLCG1 antibody (84941-1-RR, Clone: 242529A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84941-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01700-2

    Cytometric bead array standard curve of MP01700-2

    Cytometric bead array standard curve of MP01700-2, PLCG1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84941-3-PBS. Detection antibody: 84941-1-PBS. Standard: Ag27218. Range: 0.625-80 ng/mL.

Product Information

84941-1-PBS targets PLCG1 as part of a matched antibody pair:

MP01700-2: 84941-3-PBS capture and 84941-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLCG1 fusion protein Ag27218
Full Name phospholipase C, gamma 1
Calculated Molecular Weight 149 kDa
Gene Symbol PLCG1
Gene ID (NCBI) 5335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.