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Mouse CD73 Recombinant antibody, PBS Only (Detector)

CD73 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241541F5

Cat no : 84434-4-PBS

Synonyms

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CD73 Recombinant antibody (84434-4-RR)

    WB analysis of mouse brain using 84434-4-RR (same clone as 84434-4-PBS)

    mouse brain tissue was subjected to SDS PAGE followed by western blot with 84434-4-RR (5'-Nucleotidase/CD73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using CD73 Recombinant antibody (84434-4-RR)

    IF Staining of 4T1 using 84434-4-RR (same clone as 84434-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using 5'-Nucleotidase/CD73 antibody (84434-4-RR, Clone: 241541F5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84434-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01279-1

    Cytometric bead array standard curve of MP01279-1

    Cytometric bead array standard curve of MP01279-1, MOUSE CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84434-3-PBS. Detection antibody: 84434-4-PBS. Standard: Eg1389. Range: 0.625-40 ng/mL.

  • Affinity and Kinetic Characterization of 84434-4-RR

    Affinity and Kinetic Characterization of 84434-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84434-4-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

Product Information

84434-4-PBS targets CD73 as part of a matched antibody pair:

MP01279-1: 84434-3-PBS capture and 84434-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name 5' nucleotidase, ecto
Calculated Molecular Weight64kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_011851.4
Gene Symbol Nt5e
Gene ID (NCBI) 23959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).