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VISTA Recombinant antibody, PBS Only (Capture)

VISTA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241003F4

Cat no : 83919-3-PBS

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using VISTA Recombinant antibody (83919-3-RR)

    WB analysis of HeLa using 83919-3-RR (same clone as 83919-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83919-3-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83919-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00865-2

    Cytometric bead array standard curve of MP00865-2

    Cytometric bead array standard curve of MP00865-2, VISTA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83919-3-PBS. Detection antibody: 83919-2-PBS. Standard: Eg0017. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83919-3-RR

    Affinity and Kinetic Characterization of 83919-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83919-3-RR against Human VISTA were performed. The affinity constant is 6.56 nM.

Product Information

83919-3-PBS targets VISTA as part of a matched antibody pair:

MP00865-2: 83919-3-PBS capture and 83919-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name chromosome 10 open reading frame 54
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.