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VE-cadherin/CD144 Recombinant antibody, PBS Only (Capture)

VE-cadherin/CD144 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240755D8

Cat no : 83766-2-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VE-cadherin/CD144 Recombinant antibody (83766-2-RR)

    WB analysis using 83766-2-RR (same clone as 83766-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin/CD144 Recombinant antibody (83766-2-RR)

    IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin/CD144 Recombinant antibody (83766-2-RR)

    IHC staining of human placenta using 83766-2-RR (same clone as 83766-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83766-2-RR (VE-cadherin/CD144 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using VE-cadherin/CD144 Recombinant antibody (83766-2-RR)

    IF Staining of HUVEC using 83766-2-RR (same clone as 83766-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin antibody (83766-2-RR, Clone: 240755D8 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83766-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00752-1

    Cytometric bead array standard curve of MP00752-1

    Cytometric bead array standard curve of MP00752-1, VE-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-2-PBS. Detection antibody: 83766-4-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00752-2

    Cytometric bead array standard curve of MP00752-2

    Cytometric bead array standard curve of MP00752-2, VE-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83766-2-PBS. Detection antibody: 83766-3-PBS. Standard: Eg1200. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83766-2-RR

    Affinity and Kinetic Characterization of 83766-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83766-2-RR against Human VE-cadherin were performed. The affinity constant is 0.273 nM.

Product Information

83766-2-PBS targets VE-cadherin/CD144 as part of a matched antibody pair:

MP00752-1: 83766-2-PBS capture and 83766-4-PBS detection (validated in Cytometric bead array)

MP00752-2: 83766-2-PBS capture and 83766-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated Molecular Weight88 kDa
Observed Molecular Weight130 kDa, 100 kDa
GenBank Accession NumberNM_001795.5
Gene Symbol VE-cadherin
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).