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TNF-alpha Recombinant antibody, PBS Only (Capture)

TNF-alpha Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240668E6

Cat no : 83721-5-PBS

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve of MP00702-4

    Sandwich ELISA standard curve ofMP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00702-4

    Sample test of MP00702-4

    Serum of eight individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 1.76 pg/mL with a range of 0.72 - 2.78 pg/mL.

  • Cytometric bead array standard curve of MP00702-2

    Cytometric bead array standard curve of MP00702-2

    Cytometric bead array standard curve of MP00702-2, TNF-alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83721-5-PBS. Detection antibody: 83721-4-PBS. Standard: Eg0816. Range: 0.313-40 ng/mL.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83721-5-PBS targets TNF-alpha as part of a matched antibody pair:

MP00702-2: 83721-5-PBS capture and 83721-4-PBS detection (validated in Cytometric bead array)

MP00702-4: 83721-5-PBS capture and 83721-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated Molecular Weight26 kDa
GenBank Accession NumberNM_000594.4
Gene Symbol TNF alpha
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.