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HDAC1 Recombinant antibody, PBS Only (Capture)

HDAC1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240614A3

Cat no : 83624-1-PBS

Synonyms

histone deacetylase 1, HD1, GON 10, EC:3.5.1.98, EC:3.5.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC1 Recombinant antibody (83624-1-RR)

    WB analysis using 83624-1-RR (same clone as 83624-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83624-1-RR (HDAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    IF Staining of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (83624-1-RR, Clone: 240614A3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HDAC1 Recombinant antibody (83624-1-RR)

    FC experiment of HeLa using 83624-1-RR (same clone as 83624-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HDAC1 Recombinant antibody (83624-1-RR, Clone:240614A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83624-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00577-3

    Cytometric bead array standard curve of MP00577-3

    Cytometric bead array standard curve of MP00577-3, HDAC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83624-1-PBS. Detection antibody: 83624-3-PBS. Standard: Ag0256. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83624-1-RR

    Affinity and Kinetic Characterization of 83624-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83624-1-RR against Human HDAC1 were performed. The affinity constant is below 1 pM.

Product Information

83624-1-PBS targets HDAC1 as part of a matched antibody pair:

MP00577-3: 83624-1-PBS capture and 83624-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HDAC1 fusion protein Ag0256
Full Name histone deacetylase 1
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC000301
Gene Symbol HDAC1
Gene ID (NCBI) 3065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.