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Rat VEGF 164 Recombinant antibody, PBS Only (Capture/Detector)

VEGF 164 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240573B1

Cat no : 83610-1-PBS

Synonyms

Vegfa, VEGF164, Vegf, 240573B1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00598-3

    Sandwich ELISA standard curve of MP00598-3

    Sandwich ELISA standard curve of MP00598-3, Rat VEGF Recombinant Matched Antibody Pair - PBS only. 83610-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0941. 83610-2-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

  • Sample test of MP00598-3

    Sample test of MP00598-3

    Rat lung, cut into 1-2 mm pieces, was cultured for 4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 2.5 ng/mL LPS. The cell culture supernatant was removed, assayed for rat VEGF, and measured 3292.9 pg/mL. Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernatant was removed, assayed for rat VEGF, and measured 433.1 pg/mL.

  • Sample test of MP00598-3

    Sample test of MP00598-3

    Serum of eight rats was measured. The VEGF 164 concentration of one sample was determined to be 34.3 pg/mL, and the range of VEGF 164 concentration was 0 - 34.3 pg/mL.

  • Cytometric bead array standard curve of MP00598-1

    Cytometric bead array standard curve of MP00598-1

    Cytometric bead array standard curve of MP00598-1, RAT VEGF 164 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83610-2-PBS. Detection antibody: 83610-1-PBS. Standard: Eg0941. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00598-2

    Cytometric bead array standard curve of MP00598-2

    Cytometric bead array standard curve of MP00598-2, RAT VEGF 164 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83610-3-PBS. Detection antibody: 83610-1-PBS. Standard: Eg0941. Range: 0.313-40 ng/mL.

Product Information

83610-1-PBS targets VEGF 164 as part of a matched antibody pair:

MP00598-1: 83610-2-PBS capture and 83610-1-PBS detection (validated in Cytometric bead array)

MP00598-2: 83610-3-PBS capture and 83610-1-PBS detection (validated in Cytometric bead array)

MP00598-3: 83610-1-PBS capture and 83610-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name vascular endothelial growth factor A
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_001287108.1
Gene Symbol Vegfa
Gene ID (NCBI) 83785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.