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Bcl-xL Recombinant antibody, PBS Only (Detector)

Bcl-xL Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240450F2

Cat no : 83330-3-PBS

Synonyms

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00460-3

    Sandwich ELISA standard curve of MP00460-3

    Sandwich ELISA standard curve of MP00460-3, Human Bcl-xL Monoclonal Matched Antibody Pair - PBS only. 83330-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25604. 83330-3-PBS was HRP conjugated as the detection antibody. Range: 78.125-5000 pg/mL.

  • Sample test of MP00460-3

    Sample test of MP00460-3

    The mean Bcl-xL concentration was determined to be 5217.7 pg/mL in PC-12 cell extract. The mean Bcl-xL concentration was determined to be 5,486.2 pg/mL in C2C12 cell extract based on a 3.6 mg/mL extract load. The mean Bcl-xL concentration was determined to be 303.5 pg/mL in human placenta cell extract based on a 1.8 mg/mL extract load.

  • Cytometric bead array standard curve of MP00460-1

    Cytometric bead array standard curve of MP00460-1

    Cytometric bead array standard curve of MP00460-1, Bcl-xL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83330-2-PBS. Detection antibody: 83330-3-PBS. Standard: Ag25604. Range: 0.625-80 ng/mL.

Product Information

83330-3-PBS targets Bcl-xL as part of a matched antibody pair:

MP00460-1: 83330-2-PBS capture and 83330-3-PBS detection (validated in Cytometric bead array)

MP00460-3: 83330-4-PBS capture and 83330-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl-xL fusion protein Ag25604
Full Name BCL2-like 1
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.