NeutraKine® IL-10 Monoclonal antibody, PBS Only (Capture)

NeutraKine® IL-10 Monoclonal Antibody for IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, ELISA, Sandwich ELISA, Neutralization, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4F5

Cat no : 69018-1-PBS

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, NeutraKine® IL-10, TGIF



Product Information

69018-1-PBS targets NeutraKine® IL-10 as part of a matched antibody pair:

MP50035-1: 69018-1-PBS capture and 69018-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-10 protein HZ-1145
Full Name interleukin 10
Gene Symbol IL-10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer PBS Only
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

This antibody can be used to neutralize the bioactivity of IL-10.