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COXIV Monoclonal antibody, PBS Only (Capture)

COXIV Monoclonal Antibody for Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C7D2

Cat no : 60251-1-PBS

Synonyms

COX4I1, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, COX4, COX IV-1, 3C7D2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human prostate cancer using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human prostate cancer using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human pancreas using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human pancreas using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human pancreas using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human pancreas using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human heart using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human heart using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human heart using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using COXIV Monoclonal antibody (60251-1-Ig)

    IHC staining of human heart using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60251-1-Ig(COXIV antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COXIV Monoclonal antibody (60251-1-Ig)

    IF Staining of HeLa using 60251-1-Ig (same clone as 60251-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60251-1-Ig (COXIV antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60251-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50551-2

    Cytometric bead array standard curve of MP50551-2

    Cytometric bead array standard curve of MP50551-2, COXIV Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60251-1-PBS. Detection antibody: 60251-3-PBS. Standard:Ag1640. Range: 0.781-50 ng/mL.

Product Information

60251-1-PBS targets COXIV as part of a matched antibody pair:

MP50551-2: 60251-1-PBS capture and 60251-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COXIV fusion protein Ag20551
Full Name cytochrome c oxidase subunit IV isoform 1
Calculated Molecular Weight19.6 kDa
Observed Molecular Weight 17-18 kDa
GenBank Accession NumberBC021236
Gene Symbol COX IV
Gene ID (NCBI) 1327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody is specific to COX4I1 and do not cross reacts with COX4I2.