t-Plasminogen activator/tPA Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

t-Plasminogen activator/tPA Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10147-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10147-1-AP (same clone as 10147-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 10147-1-AP (same clone as 10147-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 10147-1-AP (same clone as 10147-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 10147-1-AP (same clone as 10147-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 10147-1-AP (same clone as 10147-1-PBS)

  IP result of anti-tPA (IP:10147-1-AP, 3ug; Detection:10147-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10147-1-AP (same clone as 10147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10147-1-AP (same clone as 10147-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 10147-1-AP (same clone as 10147-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10147-1-AP (tPA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10147-1-PBS in a different storage buffer formulation.

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Product Information

10147-1-PBS targets t-Plasminogen activator/tPA in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: t-Plasminogen activator/tPA fusion protein Ag0200
• Full Name: plasminogen activator, tissue
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 32-35 kDa, 65 kDa
• GenBank Accession Number: BC002795
• Gene Symbol: tPA
• Gene ID (NCBI): 5327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.