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SARS-CoV-2 S protein (126-264 aa) Polyclonal antibody

SARS-CoV-2 S protein (126-264 aa) Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 28869-1-AP

Synonyms

SARS-CoV-2 Spike Glycoprotein (126-264 aa), spike, spike protein

Validation Data Gallery

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  • ELISA experiment of Recombinant protein using SARS-CoV-2 S protein (126-264 aa) Polyclonal antib (28869-1-AP)

    ELISA experiment of Recombinant protein using 28869-1-AP

    SARS-CoV-2 Spike Antibody (28869-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28869-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28869-1-AP targets SARS-CoV-2 S protein (126-264 aa) in WB, IP, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 S protein (126-264 aa) fusion protein Ag30679
Full Name SARS-CoV-2 Spike Protein
Calculated Molecular Weight 141 kDa
GenBank Accession NumberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2881225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28869-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 126-264aa.

Publications

SpeciesApplicationTitle
WB,IP

EBioMedicine

Primate-specific BTN3A2 protects against SARS-CoV-2 infection by interacting with and reducing ACE2

Authors - Ling Xu
View Article