HumanKine® recombinant human TGF beta 3 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.01 - 0.2 ng/mL

Specific Activity

minimally 1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Publications

16

Reviews (1)

Cat no : HZ-1090

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3

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Validation Data Gallery

• 

  Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human TGF beta 3 (HZ-1090) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.14-0.75 ng/mL

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Technical Specifications

• GeneID: 7043
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.01 - 0.2 ng/mL
• Specific Activity: minimally 1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P17125
• Molecular Mass: 13 kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
• Formulation: 50 mM NaOAc pH 3.7 + 1% CHAPS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.1mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells

Background

The transforming growth factor beta-3 (TGFβ-3) protein is found throughout the body and is required for development before birth and throughout life. TGFβ-3 binds to the receptor proteins on the surface of cells. This binding triggers the transmission of signals within the cell. As part of the TGF-β a signalling pathway, the TGFβ-3 protein helps to control the growth and cell proliferation. The TGFβ-3 protein is especially abundant in skeletal muscles and plays a key role in their development. The protein is also involved in blood vessels formation, regulation of bone growth, and wound healing (PMID: 25835445; 23824657)

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3

Publications

Species	Title
rat	Biology (Basel); Myogenic Differentiation and Immunomodulatory Properties of Rat Adipose-Derived Mesenchymal Stem/Stromal Cells; Authors - Sai Koung Ngeun
human	Biochem J; Soluble CD109 binds TGF-β and antagonizes TGF-β signalling and responses.; Authors - CarterLi
human	Front Bioeng Biotechnol; Mechano-Hypoxia Conditioning of Engineered Human Meniscus.; Authors - Alexander R A Szojka
human	Biol Reprod; Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.; Authors - YangGao
human	PLoS One; Oxygen tension is a determinant of the matrix-forming phenotype of cultured human meniscal fibrochondrocytes.; Authors - Adetola BAdesida
mouse	PLoS One; YBX1 is a modulator of MIA/CD-RAP-dependent chondrogenesis.; Authors - RainerSchmid

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (07-27-2022)

crucial supplement

• Applications: Cell culture
• Cell Tissue Type: Dopaminergic Neurons