HumanKine® recombinant human TGF beta 2 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.018-0.18 ng/mL

Specific Activity

minimally 2.00 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

8

Cat no : HZ-1092

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

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Validation Data Gallery

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  Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human TGF beta 2 (HZ-1092) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.018-0.18 ng/mL.

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Technical Specifications

• GeneID: 7042
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.018-0.18 ng/mL
• Specific Activity: minimally 2.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P27090
• Molecular Mass: 13 kDa reduced, 23 kDa non-reduced, homodimer, non-glycosylated
• Formulation: 50 mM NaOAc pH 3.7, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 20% ethanol + 50 mM NaOAc + 75 mM HOAc. Gently swirl or tap vial to mix.

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells

Background

Transforming growth factor beta-2 (TGFB2) belongs to the TGF-beta family. TGFB2 is a secreted protein known as a cytokine that plays a vital role during embryonic development (PMID 19289080). TGFB2 regulates cell proliferation, differentiation, adhesion, migration by transducing the signal through transmembrane receptors type I and type II (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins (PMID 18313409). Disruption of the TGFB/SMAD pathway has been implicated in many cancers types. TGFB2 has suppressive effects on interleukin-2 dependent T-cell growth (PMID 12064833). The TGFβ2 protein plays a role in the blood vessels formation, the regulation of muscle tissue and body fat development. TGFβ-2 is especially abundant in tissues related to the skeleton, where it helps regulate bone growth and form the extracellular matrix in the spaces between cells.

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

Publications

Species	Title
mouse,human	J Cell Mol Med; FGF2 antagonizes aberrant TGFβ regulation of tropomyosin: role for posterior capsule opacity.; Authors - EriKubo
human	Biochem J; Soluble CD109 binds TGF-β and antagonizes TGF-β signalling and responses.; Authors - CarterLi
human	Biol Reprod; Constitutive activation of transforming growth factor Beta receptor 1 in the mouse uterus impairs uterine morphology and function.; Authors - YangGao
human	Invest Ophthalmol Vis Sci; Aldose Reductase Mediates Transforming Growth Factor β2 (TGF-β2)-Induced Migration and Epithelial-To-Mesenchymal Transition of Lens-Derived Epithelial Cells.; Authors - Kun-CheChang
	Front Oncol; Association of the TGFβ gene family with microenvironmental features of gastric cancer and prediction of response to immunotherapy; Authors - Bangling Han
	BMC Genomics; Transcriptome exploration of ferroptosis-related genes in TGFβ- induced lens epithelial to mesenchymal transition during posterior capsular opacification development; Authors - Cong Fan