HumanKine® recombinant human SCF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

15-85 ng/mL EC50

Specific Activity

>2.00 x 10⁵ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

15

Cat no : HZ-1024

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

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Validation Data Gallery

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  Purity of recombinant human SCF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human SCF (HZ-1024) stimulates dose-dependent proliferation of the MO7e human megakaryoblastic leukemia cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant SCF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 15-85 ng/mL​.

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Technical Specifications

• GeneID: 4254
• Species: Human
• Expression: HEK293
• Activity(Primary): 15-85 ng/mL EC50
• Specific Activity: >2.00 x 10^5 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P21583
• Molecular Mass: 30 to 45 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Publications

Species	Title
human	Blood; LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo.; Authors - Y TerryLee
human	J Exp Med; Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.; Authors - AndrewVolk
human	Mol Cancer Ther; IMGN779, a Novel CD33-Targeting Antibody-Drug Conjugate with DNA Alkylating Activity, Exhibits Potent Antitumor Activity in Models of AML.; Authors - YelenaKovtun
human	Stem Cells; Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines.; Authors - Niels-BjarneWoods
human	ACS Biomater Sci Eng; Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.; Authors - Lidan Chen
human	Oncotarget; Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.; Authors - JingLi