• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human SCF protein

Activity

15-85 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1024

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor



Technical Specifications

GeneID 4254
Species Human
Expression HEK293
Activity 15-85 ng/mL EC50
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP21583
Molecular Mass 35 to 45 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Publications

SpeciesTitle
human

Haematologica

CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.

Authors - Evgeniya Angelova
human

ACS Biomater Sci Eng

Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.

Authors - Lidan Chen
human

Blood Res

Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.

Authors - So-JungKim
human

PLoS One

A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.

Authors - Y TerryLee
human

Sci Rep

A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.

Authors - Dezhi Zhou

iScience

Use of dual-electron probes reveals the role of ferritin as an iron depot in ex vivo erythropoiesis.

Authors - Maria A Aronova