HumanKine® recombinant human SCF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

15-85 ng/mL EC50

Species Reactivity

human

Purity

>95%

Publications

13

Cat no : HZ-1024

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human SCF (HZ-1024) stimulates dose-dependent proliferation of the MO7e human megakaryoblastic leukemia cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells were treated with increasing concentrations of GMP recombinant SCF for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 15-85 ng/mL​.

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Technical Specifications

• GeneID: 4254
• Species: Human
• Expression: HEK293
• Activity: 15-85 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P21583
• Molecular Mass: 35 to 45 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Publications

Species	Title
human	Haematologica; CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.; Authors - Evgeniya Angelova
human	ACS Biomater Sci Eng; Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.; Authors - Lidan Chen
human	Blood Res; Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.; Authors - So-JungKim
human	PLoS One; A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.; Authors - Y TerryLee
human	Sci Rep; A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.; Authors - Dezhi Zhou
	iScience; Use of dual-electron probes reveals the role of ferritin as an iron depot in ex vivo erythropoiesis.; Authors - Maria A Aronova