HumanKine® recombinant human Noggin protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

1.5-15 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

12

Reviews (1)

Cat no : HZ-1118

Synonyms

NOG, noggin, SYM1, SYNS1

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Validation Data Gallery

• 

  Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 1.5-15 ng/mL.

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Technical Specifications

• GeneID: 9241
• Species: Human
• Expression: HEK293
• Activity(Primary): 1.5-15 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: Q13253
• Molecular Mass: 65 kDa, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

HumanKine® Noggin Human Cell Expressed Glycosylated Disulfide-linked Homodimer

Background

Noggin is an inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. It is essential for cartilage morphogenesis and joint formation. Noggin inhibits chondrocyte differentiation through its interaction with GDF5 and GDF6 (PMID: 29497255; 29167826; 29094227; 28842965).

Synonyms

NOG, noggin, SYM1, SYNS1

Publications

Species	Title
human	Nat Methods; Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.; Authors - YukiYamamoto
human	Cell Stem Cell; Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro.; Authors - Anindita Sarkar
mouse	Nat Commun; Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells; Authors - Yang Sun
human	Biomaterials; Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo.; Authors - Satoshi Ikeo
human	Curr Biol; Human-specific regulation of neural maturation identified by cross-primate transcriptomics; Authors - Sara B Linker
human	Stem Cell Reports; Directed Induction of Functional Multi-ciliated Cells in Proximal Airway Epithelial Spheroids from Human Pluripotent Stem Cells.; Authors - SatoshiKonishi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lisa (Verified Customer) (08-11-2022)

The cytokine works well, with results comparable with the ones observed using the same product from other companies.

• Applications: Cell culture
• Cell Tissue Type: hiPSCs-derived neurons