HumanKine® recombinant human LEFTY-1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

Typically ≤ 2.5 µg/mL

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Cat no : HZ-1109

Synonyms

LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B

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Validation Data Gallery

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  Purity of recombinant human Lefty-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  The activity of Lefty-1 was determined by the dose-dependent inhibition of proliferation of the Mv1Lu cell line (mink lung epithelial cells) using ProMega CellTiter96® aqueous Non-radioactive cell proliferation assay.

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Technical Specifications

• GeneID: 10637
• Species: Human
• Expression: HEK293
• Activity(Primary): Typically ≤ 2.5 µg/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: O75610
• Molecular Mass: 43 kDa reduced, 40 and 43 kDa non-reduced, monomer, glycosylated
• Formulation: 10 mM Tris pH 7.4 + 450 mM NaCl + 1% CHAPS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

LEFTY-1 is a member of the TGF-beta family of proteins that plays a role in left-right asymmetry determination of organ systems during development. LEFTY-1 regulates transforming growth factor, mitogen-activated protein kinase and other signaling pathways, and is considered to have a potential anti-inflammatory function (PMID: 29286065; 26316705; 10500184; 9708731)

Synonyms

LEFTB, LEFTY1, LEFTYB, Protein lefty 1, Protein lefty B

Publications

Species	Title
human	Stem Cells Dev; Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.; Authors - ZhilaKhalkhali-Ellis