- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IL-4 protein
Activity(Primary)
0.07-0.4 ng/mL EC50
Specific Activity
>5.00 x 106 IU/mg
Species Reactivity
human,mouse,pig
Purity
>95%
Cat no : HZ-1004
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
Validation Data Gallery
Technical Specifications
GeneID | 3565 |
Species | Human |
Expression | HEK293 |
Activity(Primary) | 0.07-0.4 ng/mL EC50 |
Specific Activity | >5.00 x 106 IU/mg |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P05112 |
Molecular Mass | 14 and 19 kDa, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse,pig |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival (PMID: 24489573;3049907;21663408)
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
Publications
Species | Title |
---|---|
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | |
J Exp Med Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors. | |
Biomaterials In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance. | |
J Neuroinflammation Monocarboxylate transporter 1 promotes classical microglial activation and pro-inflammatory effect via 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3. | |
J Virol HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms. | |
Immunol Cell Biol Regulation of human Th9 differentiation by type I interferons and IL-21. |