HumanKine® recombinant human IL-3 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.4-2.0 ng/mL

Specific Activity

minimally 5.0 x 10⁵ IU/mg

Species Reactivity

human

Purity

>95%

Publications

15

Reviews (2)

Cat no : HZ-1074

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

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Validation Data Gallery

• 

  Purity of recombinant human IL-3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human IL-3 (HZ-1074) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.4-2.0 ng/mL

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Technical Specifications

• GeneID: 3562
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.4-2.0 ng/mL
• Specific Activity: minimally 5.0 x 10^5 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P08700
• Molecular Mass: 17 to 50 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1xPBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4. Gently swirl or tap vial to mix.

Background

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

Publications

Species	Title
human	Blood; LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo.; Authors - Y TerryLee
human	J Exp Med; Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.; Authors - AndrewVolk
	Acta Pharmacol Sin; VEGFD/VEGFR3 signaling contributes to the dysfunction of the astrocyte IL-3/microglia IL-3Rα cross-talk and drives neuroinflammation in mouse ischemic stroke; Authors - Shuai Wang
human	Mol Cancer Ther; IMGN779, a Novel CD33-Targeting Antibody-Drug Conjugate with DNA Alkylating Activity, Exhibits Potent Antitumor Activity in Models of AML.; Authors - YelenaKovtun
human	Oncotarget; Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.; Authors - JingLi
human	Eur J Pharmacol; Ginsenoside Rg1 promotes fetal hemoglobin production in vitro: A potential therapeutic avenue for β-thalassemia; Authors - Dong-Ling Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (12-11-2024)

We use IL3 to maintain our macrophage precursor production from our factories using human iPSCs following the Haenseler protocol.

• Applications: Cell culture
• Cell Tissue Type: Human iPSC-derived factories for macrophage precursor production

FH

Romane (Verified Customer) (12-06-2024)

Cytokine used for embryoid bodies production of immune cells, which was successful.

• Applications: Cell culture
• Cell Tissue Type: Human derived iPSCs