HumanKine® recombinant human IL-3 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.4-2.0 ng/mL EC50

Species Reactivity

human

Purity

>95%

Publications

13

Cat no : HZ-1074

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human IL-3 (HZ-1074) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.4-2.0 ng/mL

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Technical Specifications

• GeneID: 3562
• Species: Human
• Expression: HEK293
• Activity: 0.4-2.0 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P08700
• Molecular Mass: 17 to 40 kDa, monomer, glycosylated
• Formulation: 1xPBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile Ix PBS.

Background

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

Publications

Species	Title
human	Haematologica; CD123 expression patterns and selective targeting with a CD123-specific antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia.; Authors - Evgeniya Angelova
human	Genet Mol Res; Impact of BMMSCs from different sources on proliferation of CD34⁺ cells.; Authors - R F Liu
human	Blood Res; Generation of hematopoietic stem cells from human embryonic stem cells using a defined, stepwise, serum-free, and serum replacement-free monolayer culture method.; Authors - So-JungKim
human	J Toxicol Sci; Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.; Authors - Tatsuya Ikuno
human	Bioelectromagnetics; Evaluation of the Effects of Exposure to Power-Frequency Magnetic Fields on the Differentiation of Hematopoietic Stem/Progenitor Cells Using Human-Induced Pluripotent Stem Cells.; Authors - Masayuki Takahashi
human	PLoS One; A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors.; Authors - Y TerryLee