HumanKine® recombinant human IL-28A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.01-0.06 ng/mL

Species Reactivity

human

Purity

>95%

Publications

2

Cat no : HZ-1235

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

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Validation Data Gallery

• 

  Purity of recombinant human IL-28A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IL-28A (HZ-1235) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.01-0.06 ng/mL.

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Technical Specifications

• GeneID: 282616
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.01-0.06 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: Q8IZJ0
• Molecular Mass: 22 kDa reduced, 19 and 35 kDa non-reduced, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL28A is a type III IFN, which shares many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. It interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). IL28A is believed to play a significant role in the antiviral immune defense in the intestinal epithelium (PMID: 20712453;12469119;16539846).

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

Publications

Species	Title
	Antiviral Res; Antiviral candidates against the hepatitis E virus (HEV) and their combinations inhibit HEV growth in in vitro.; Authors - Takashi Nishiyama
human	J Virol Methods; Screening of novel drugs for inhibiting hepatitis E virus replication.; Authors - Takashi Nishiyama