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Animal-free Recombinant Human IL-271 Publications

Catalog number: HZ-1275

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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IL-27 activity assay

The specific activity was determined by the dose-dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages as described in Fakruddin, J.M., et al. Blood 109:1841.

IL-27 purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
246778
Species:
Human
Expression:
HEK293
Activity:
Typically ≤12 ng/mL EC50
Purity:
>95%
Molecular Mass:
25 and 30 kDa heterodimer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human IL-27 is expressed in human 293 cells as a non-covalently bonded heterodimer cytokine composed of IL27p28 (25 kDa) and glycosylated EBI3 (30 kDa). Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation. Cytokines produced in non-human expression systems may have an artificial linker, a tag, and/or not human-like posttranslational modifications.



Background:

IL-27 is a heterodimeric cytokine composed of two subunits: IL-27a and IL-27b. IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases (PMID: 22608254;16081786;16670296;16906167)



Synonyms:
IL27, IL 27



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