HumanKine® recombinant human IL-23 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

1.25-6.5 ng/mL in murine splenocytes

Species Reactivity

human,mouse

Purity

>95%

Publications

13

Cat no : HZ-1254

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF

---

Validation Data Gallery

• 

  Purity of recombinant IL-23 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with coomassie blue.

• 

  Recombinant human IL-23 (HZ-1254) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.2-1.2 ng/mL.

• 

  Proteintech IL-23 (HZ-1254) demonstrates more 20-fold decrease in EC50 and much higher fold induction compared to leading competitor A. Recombinant human IL-23 stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.2-1.2 ng/mL.

• 

  Recombinant human IL-23 (Cat no: HZ-1254) induces dose-dependent release of IL-17 in murine splenocytes. Murine splenocytes cells were treated with increasing concentration of recombinant IL-23 for 72 hours before supernatant collection. The supernatant was tested for IL-17 using an IL-17 ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 is less than 1.25-6.5 ng/mL

---

Technical Specifications

• GeneID: 51561,3593
• Species: Human
• Expression: HEK293
• Activity(Primary): 1.25-6.5 ng/mL in murine splenocytes
• Activity(Secondary): 0.2-1.2 ng/mL in HEK293 reporter cell line
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: Q9NPF7
• Molecular Mass: 20 and 42 kDa reduced, 53 kDa non-reduced, heterodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

HumanKine® IL-23 100x More Potent For Induction of Human Th17 Cells

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Background

Interleukin 23 (IL-23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL-23 (the IL-23 receptor) has been identified and is composed of IL-12Rβ1 and IL-23R. IL-23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL-6. The main biological effects of IL-23 identified initially are stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL-23 acts also as an end-stage effector cytokine through direct action on macrophages (PMID: 16393998;15657292;12023369;12610626)

Synonyms

IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL23, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF

Publications

Species	Title
human	Ann Rheum Dis; JAK selectivity and the implications for clinical inhibition of pharmacodynamic cytokine signalling by filgotinib, upadacitinib, tofacitinib and baricitinib.; Authors - Paqui G Traves
human	Cell Rep; Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.; Authors - YusukeEndo
human	Br J Pharmacol; The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation; Authors - Yanhong Pan
human	J Mol Cell Cardiol; Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.; Authors - MakikoOhshima
human	J Immunol; The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.; Authors - Raymond YYu
human	Sci Rep; Functional defects in CD4+ CD25high FoxP3+ regulatory cells in ankylosing spondylitis.; Authors - HuifangGuo