HumanKine® recombinant human IL-1 beta protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.8-4.0 ng/mL in an HEK293 reporter cell line, Typically ≤ 0.05 ng/mL in D10.G4.1 cells

Specific Activity

minimally 1.00 x 10⁶ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

43

Cat no : HZ-1164

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Validation Data Gallery

• 

  Purity of recombinant human IL-1 beta was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  ecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. D10.G4.1 cells were treated with increasing concentrations of recombinant IL-1 beta for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.

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  Recombinant human IL-1 beta (Cat no: HZ-1164) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 0.8-4.0 ng/mL ng/mL EC50.

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Technical Specifications

• GeneID: 3553
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.8-4.0 ng/mL in an HEK293 reporter cell line, Typically ≤ 0.05 ng/mL in D10.G4.1 cells
• Activity(Secondary): 0.8-4.0 ng/mL in an HEK293 reporter cell line
• Specific Activity: minimally 1.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01584
• Molecular Mass: 20 and 23 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1b, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Publications

Species	Title
human	Cell Rep; Obesity Drives Th17 Cell Differentiation by Inducing the Lipid Metabolic Kinase, ACC1.; Authors - YusukeEndo
human	Aging Cell; Age-related sensitivity to endotoxin-induced liver inflammation: Implication of inflammasome/IL-1β for steatohepatitis.; Authors - Ki WungChung
mouse	Food Funct; Urolithin B reduces cartilage degeneration and alleviates osteoarthritis by inhibiting inflammation; Authors - Hong Xue
mouse	Mol Ther Nucleic Acids; AP-1 Oligodeoxynucleotides Reduce Aortic Elastolysis in a Murine Model of Marfan Syndrome.; Authors - RawaArif
human	J Mol Cell Cardiol; Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.; Authors - MakikoOhshima
mouse	J Immunol; IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures.; Authors - LarsBlom