- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human IFN alpha 2A protein
Activity(Primary)
Typically ≤ 0.06 ng/mL
Specific Activity
minimally 1.00 x 107 IU/mg
Species Reactivity
human
Purity
>95%
Cat no : HZ-1066
Synonyms
IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A
Validation Data Gallery
Technical Specifications
| GeneID | 3440 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | Typically ≤ 0.06 ng/mL |
| Specific Activity | minimally 1.00 x 107 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P01563 |
| Molecular Mass | 18 to 22 kDa reduced, 14 to 19 kDa non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761).
Synonyms
IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A
Publications
| Species | Title |
|---|---|
Ann Rheum Dis Parsing multiomics landscape of activated synovial fibroblasts highlights drug targets linked to genetic risk of rheumatoid arthritis. | |
J Am Chem Soc Using an Old Drug to Target a New Drug Site: Application of Disulfiram to Target the Zn-Site in HCV NS5A Protein. | |
Int J Mol Sci Interferon-Induced Transmembrane Protein 1 (IFITM1) Promotes Distant Metastasis of Small Cell Lung Cancer. | |
Cell Mol Immunol BCR-ABL-specific CD4+ T-helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells. | |
ACS Infect Dis The Nonstructural Protein of Guertu Virus Disrupts Host Defenses by Blocking Antiviral Interferon Induction and Action. | |
Cancer Sci Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Christine (Verified Customer) (12-14-2021) | I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFNalpha2a. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN beta (cat number HZ-1298) at the same time and the results were also very good, identical.
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