HumanKine® recombinant human HGH protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.05-0.5 ng/mL

Species Reactivity

human,rat

Purity

>95%

Publications

3

Cat no : HZ-1007

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin

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Validation Data Gallery

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  Purity of recombinant human HGH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human HGH (HZ-1007) stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.5 ng/mL​.

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  Proteintech HGH (HZ-1007) demonstrates equivalent induction of proliferation and a 2-fold lower EC50 compared to leading competitors. Recombinant human HGH stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.02-0.120 ng/mL​.

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Technical Specifications

• GeneID: 2688
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.05-0.5 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01241
• Molecular Mass: 22 kDa reduced, 20 kDa non-reduced, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Human Growth hormone (HGH), also known as somatotropin, is an integral mitogen for development. It is synthesized, stored and secreted by somatotropic cells within the anterior pituitary gland (PMID 13975314). HGH stimulates growth, cell reproduction, and cell regeneration in humans and other animals (PMID 7079756). HGH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues (PMID 8203982, 16332656).

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin

Publications

Species	Title
human	J Biol Chem; Pyruvate dehydrogenase complex (PDC) subunits moonlight as interaction partners of phosphorylated STAT5 in adipocytes and adipose tissue.; Authors - Allison JRichard
rat	Protein Expr Purif; High-level expression and purification of recombinant human growth hormone produced in soluble form in Escherichia coli.; Authors - Zdenko Levarski
	Adv Sci (Weinh); Investigating Novel Therapeutic Approaches for Idiopathic Short Stature: Targeting siRNA and Growth Hormone Delivery to the Growth Plate Using Exosome Nanoparticles; Authors - Jinghong Yuan