- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human HGF protein
Activity(Primary)
5-25 ng/mL
Specific Activity
minimally 2.50 x 105 IU/mg
Species Reactivity
human,monkey,mouse,canine,rat,zebrafish
Purity
>95%
Cat no : HZ-1084
Synonyms
F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Validation Data Gallery
Technical Specifications
| GeneID | 3082 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 5-25 ng/mL |
| Specific Activity | minimally 2.50 x 105 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P14210 |
| Molecular Mass | 85 kDa reduced, 70 kDa non-reduced, single chain, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,monkey,mouse,canine,rat,zebrafish |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. It is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin (PMID 1530787, 2531289). HGF exerts a variety of effects on several cells, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities (PMID: 1846706; 15070743; 1383237; 1827664).
Synonyms
F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Publications
| Species | Title |
|---|---|
J Cell Biol Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells. | |
Pharmacol Res The natural product berberine synergizes with osimertinib preferentially against MET-amplified osimertinib-resistant lung cancer via direct MET inhibition | |
Toxicol Sci In Silico Modeling for the Prediction of Dose and Pathway-Related Adverse Effects in Humans From In Vitro Repeated-Dose Studies. | |
Toxicol Lett Tools for studying the metabolism of new psychoactive substances for toxicological screening purposes - A comparative study using pooled human liver S9, HepaRG cells, and zebrafish larvae. | |
Anal Bioanal Chem Metabolic fate of desomorphine elucidated using rat urine, pooled human liver preparations, and human hepatocyte cultures as well as its detectability using standard urine screening approaches. | |
J Pharm Biomed Anal Pooled human liver preparations, HepaRG, or HepG2 cell lines for metabolism studies of new psychoactive substances? A study using MDMA, MDBD, butylone, MDPPP, MDPV, MDPB, 5-MAPB, and 5-API as examples. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Daman (Verified Customer) (09-11-2023) | We originally sourced our HGF from another company but they had poor availability, not only did this arrive quickly it was considerably cheaper and worked just as well.
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