HumanKine® recombinant human GM-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.08-0.8 ng/mL

Specific Activity

minimally 6.00x10⁶ IU/mg

Species Reactivity

human,mouse,pig

Purity

>95%

Publications

29

Reviews (1)

Cat no : HZ-1002

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

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Validation Data Gallery

• 

  Purity of recombinant human GM-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  The activity was determined by dose-dependent stimulation of proliferation in human TF-1 cells (human erythroleukemic indicator cell line) measured using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 1437
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.08-0.8 ng/mL
• Specific Activity: minimally 6.00x10^6 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P04141
• Molecular Mass: 15 to 36 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Native Glycosylation Is Important For Biological Function and Activity of Recombinant Human Proteins

Time-Saving Dendritic Cell Generation No Medium Change - Less Reagents With HumanKine® GM-CSF and HumanKine IL4

Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim

Publications

Species	Title
	ACS Nano; Engineered Norovirus-Derived Nanoparticles as a Plug-and-Play Cancer Vaccine Platform; Authors - Peng Zheng
human	Blood; GM-CSF-dependent pSTAT5 sensitivity is a feature with therapeutic potential in chronic myelomonocytic leukemia.; Authors - EricPadron
mouse	Small Methods; Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy; Authors - Shilin Li
human	J Exp Med; Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors.; Authors - Benjamin Fournier
mouse	ACS Appl Mater Interfaces; Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform; Authors - Xianglong Yu
	Biomaterials; In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance.; Authors - Yue Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maria Fernanda (Verified Customer) (12-19-2023)

Used as coating reagent for an ELISA

• Applications: Other