HumanKine® recombinant human G-CSF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.009-0.05 ng/mL

Specific Activity

minimally 1.00 x 10⁸ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

12

Cat no : HZ-1207

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

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Validation Data Gallery

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  Purity of recombinant human G-CSF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human G-CSF (HZ-1207) stimulates dose-dependent proliferation of the M-NFS-60 Mouse Myeloid Leukemia indicator cells line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M-NFS-60 cells were treated with increasing concentrations of recombinant human G-CSF for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 value range is 0.009-0.05 ng/mL.

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Technical Specifications

• GeneID: 1440
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.009-0.05 ng/mL
• Specific Activity: minimally 1.00 x 10^8 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P09919
• Molecular Mass: 21 to 25 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Granulocyte colony-stimulating factor (G-CSF), also known as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils and trophoblast cells. G-CSF exerts its effects by binding to its receptor G-CSFR, in turn activating the JAK/STAT and Ras/Raf/MAP kinase pathways (PMID 9015216, 7509213, 9933603). G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy (PMID 7524753, 3264199). Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis (PMID 18524991).

Synonyms

C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin

Publications

Species	Title
mouse	Cell Stem Cell; Robo4 cooperates with CXCR4 to specify hematopoietic stem cell localization to bone marrow niches.; Authors - StephanieSmith-Berdan
mouse	J Mol Cell Biol; Protective effects of human umbilical cord mesenchymal stem cell-derived conditioned medium on ovarian damage.; Authors - Liming Hong
mouse	Cell Prolif; Overproduced bone marrow neutrophils in collagen-induced arthritis are primed for NETosis: An ignored pathological cell involving inflammatory arthritis.; Authors - Danyi Xu
human	J Virol; HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.; Authors - RémiPlanès
human	J Cell Mol Med; M2 macrophage-derived G-CSF promotes trophoblasts EMT, invasion and migration via activating PI3K/Akt/Erk1/2 pathway to mediate normal pregnancy.; Authors - Jinli Ding
human	PLoS One; HIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway.; Authors - NawalBen Haij