HumanKine® recombinant human FGF-7 (KGF) protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

4-20 ng/mL

Specific Activity

minimally 2.00 x 10⁵ IU/mg

Species Reactivity

human,monkey,mouse

Purity

>95%

Publications

3

Cat no : HZ-1100

Synonyms

FGF 7, FGF7, FGF-7, Fibroblast growth factor 7, HBGF 7, Keratinocyte growth factor, KGF

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Validation Data Gallery

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  Purity of recombinant human FGF-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human FGF-7 (HZ-1100) Stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 ells were treated with increasing concentrations of recombinant human FGF-7 for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 4-20 ng/mL.

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Technical Specifications

• GeneID: 2252
• Species: Human
• Expression: HEK293
• Activity(Primary): 4-20 ng/mL
• Specific Activity: minimally 2.00 x 10^5 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P21781
• Molecular Mass: 17 and 25 to 30 kDa reduced, 17 and 24 to 29 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,monkey,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Fibroblast growth factor 7 (FGF-7), also known as keratinocyte growth factor (KGF), belongs to the FGF family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (PMID 11276432, 15863033). FGF-7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes (PMID 15327889).

Synonyms

FGF 7, FGF7, FGF-7, Fibroblast growth factor 7, HBGF 7, Keratinocyte growth factor, KGF

Publications

Species	Title
human	J Invest Dermatol; Increased KGF expression promotes fibroblast activation in a double paracrine manner resulting in cutaneous fibrosis.; Authors - JohannaCanady
mouse	Stem Cell Res Ther; Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration.; Authors - Naoya Kawakita
human	Phytomedicine; Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion; Authors - Junwei Gao