HumanKine® recombinant human FGF-4 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Reviews (1)

Cat no : HZ-1218

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

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Validation Data Gallery

• 

  Purity of recombinant human FGF-4 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

• 

  Recombinant human FGF-4 (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-4 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 6-30ng/mL.

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Technical Specifications

• GeneID: 2249
• Species: Human
• Expression: HEK293
• Activity(Primary): ≤ 1.25 ng/mL in Balb/c 3T3 cells. 6-30 ng/mL in NIH 3T3 cells in defined media
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P11403
• Molecular Mass: 17 and 27 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4. Gently swirl or tap vial to mix.

Background

Fibroblast growth factor-4 (FGF-4) plays an important physiologically role in the self-renewal of human embryonic stem cells (PMID 21990129). It also promotes stem cell proliferation and may aid in differentiation. Xeno-free Recombinant Human FGF-4 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa.

Synonyms

FGF 4, FGF4, FGF-4, fibroblast growth factor 4, HBGF 4, HST, HST 1, HSTF 1, HSTF1, K FGF, KFGF, KS3, Transforming protein KS3

Publications

Species	Title
mouse	J Cell Commun Signal; Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice.; Authors - Yin Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hariam (Verified Customer) (12-06-2024)

Good product, we use it for human iPSC maintenance. Works great

• Applications: Cell culture
• Cell Tissue Type: Induced Pluripotent Stem Cells