• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Wnt3A protein- GMP grade

EC50

25-125 ng/mL EC50

Species Reactivity

human,mouse

Purity

>90%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1296-GMP

Synonyms

Wnt 3a, WNT3A, wingless-type MMTV integration site family member 3A



Technical Specifications

GeneID 89780
Species Human
Expression HEK293
EC50 25-125 ng/mL EC50
Purity >90%
Endotoxin<0.1 EU/µg
Accession NumberP56704
Molecular Mass 38-42 kDa
Formulation 1x PBS pH 7.4 + 500mM NaCl +1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)

Synonyms

Wnt 3a, WNT3A, wingless-type MMTV integration site family member 3A