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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TNF alpha protein- GMP grade

EC50

0.002-0.026 ng/mL

Specific Activity

minimally 7.00 x 106 IU/mg, typically 1.59 x 107 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1014-GMP

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

Validation Data Gallery

  • Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human TNF alpha was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.

    GMP Recombinant human TNF alpha (HZ-1014-GMP) demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.002-0.026 ng/mL.

Technical Specifications

GeneID 7124
Species Human
Expression HEK293
EC50 0.002-0.026 ng/mL
Specific Activityminimally 7.00 x 106 IU/mg, typically 1.59 x 107 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP01375
Molecular Mass 17-20 kDa reduced and non-reduced, non-disulfide bonded homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane-bound protein and free soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced mostly by activated macrophages, CD4+ lymphocytes, NK cells, neutrophils, eosinophils, and neurons. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation (PMID: 20194223).

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor