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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TGF beta 3 protein- GMP grade

EC50

0.01 - 0.2 ng/mL

Specific Activity

minimally 1.00 x 107 IU/mg, typically 1.81 x 107 IU/mg

Species Reactivity

human,mouse

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1090-GMP

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3

Validation Data Gallery

  • Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human TGF beta 3 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human TGF beta 3 (HZ-1090-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is ≤ 0.75 ng/mL

    GMP Recombinant human TGF beta 3 (HZ-1090-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 3 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is ≤ 0.75 ng/mL

Technical Specifications

GeneID 7043
Species Human
Expression HEK293
EC50 0.01 - 0.2 ng/mL
Specific Activityminimally 1.00 x 107 IU/mg, typically 1.81 x 107 IU/mg
Purity > 95%
Endotoxin< 0.1 EU/µg
Accession NumberP10600
Molecular Mass 13 kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
Formulation 50 mM NaOAc pH 3.7 + 1% CHAPS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein 0.1 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

The transforming growth factor beta-3 (TGFβ-3) protein is found throughout the body and is required for development before birth and throughout life. TGFβ-3 binds to the receptor proteins on the surface of cells. This binding triggers the transmission of signals within the cell. As part of the TGF-β a signalling pathway, the TGFβ-3 protein helps to control the growth and cell proliferation. The TGFβ-3 protein is especially abundant in skeletal muscles and plays a key role in their development. The protein is also involved in blood vessels formation, regulation of bone growth, and wound healing (PMID: 25835445; 23824657)

Synonyms

ARVD, FLJ16571, TGF beta 3, TGF beta3, TGFB3