HumanKine® recombinant human Pleiotrophin (PTN) protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

100-500 ng/mL

Species Reactivity

human,rat

Purity

> 95%

Cat no : HZ-1278-GMP

Synonyms

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN

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Validation Data Gallery

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  Purity of recombinant human PTN was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP-grade recombinant human PTN (HZ-1278) stimulates dose-dependent proliferation of the THP-1 monocyte cell line. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant PTN for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 120-650 ng/mL​.

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Technical Specifications

• GeneID: 5764
• Species: Human
• Expression: HEK293
• EC50: 100-500 ng/mL
• Purity: > 95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P21246
• Molecular Mass: 18-21 kDa reduced, 17 to 20 kDa non-reduced, monomer, non-glycosylated
• Formulation: 1 x PBS, See Certificate of Analysis for details
• Species Reactivity: human,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Pleiotrophin is a secreted growth factor that induces neurite outgrowth being mitogenic for fibroblasts, epithelial, and endothelial cells. It binds anaplastic lymphoma kinase (ALK) inducing MAPK pathway activation, as an important step in the anti-apoptotic signaling of PTN and cell proliferation (PMID:1768439; 1733956; 11278720)

Synonyms

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN