HumanKine® recombinant human LIF protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.045-0.25 ng/mL

Specific Activity

minimally 3.50 x 10⁶ IU/mg, typically 9.50 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1292-GMP

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI

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Validation Data Gallery

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  Purity of recombinant human LIF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human LIF (HZ-1292-GMP) dose-dependently inhibits growth of the M1 cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. M1 cells were treated with increasing concentrations of recombinant LIF for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.045-0.25 ng/mL​.

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Technical Specifications

• GeneID: 3976
• Species: Human
• Expression: HEK293
• EC50: 0.045-0.25 ng/mL
• Specific Activity: minimally 3.50 x 10^6 IU/mg, typically 9.50 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P15018
• Molecular Mass: 35-55 kDa reduced and non-reduced, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

LIF is a pleiotropic cytokine with a variety of roles. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface (PMID: 29620145; 29391217; 29271384)

Synonyms

CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI