HumanKine® recombinant human IL-8 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

100-500 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1318

Synonyms

CXCL8, CXCL8SCYB8, interleukin 8, IL8, AMCF-I, C-X-C motif chemokine 8, NAF, NAP1, NCF GCP1, GCP-1,TSG-1, K60, LAI, LECT, LUCT, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, Neutrophil-activating protein 1

---

Validation Data Gallery

• 

  Purity of recombinant human IL-8 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

• 

  Recombinant human IL-8 (HZ-1318) stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

• 

  Proteintech IL-8 (HZ-1318) demonstrates a 2.5-fold decrease in EC50 and a greater fold induction compared to leading competitors. Recombinant human IL-8 stimulates dose-dependent proliferation of the THP-1 human monocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. THP-1 cells were treated with increasing concentrations of recombinant IL-8 for 144 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.

---

Technical Specifications

• GeneID: 3576
• Species: Human
• Expression: HEK293
• Activity(Primary): 100-500 ng/mL EC50
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P10145
• Molecular Mass: 12 kDa
• Formulation: 1x PBS
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4.

Background

IL-8 is a chemoattractant cytokine that signals through the CXCR1 and CXCR2 receptors to attract and activate neutrophils to sites of inflammation. It is secreted by multiple types of cells, including monocytes, neutrophils, tumor, epithelial, endothelial, and fibroblasts. IL-8 signaling activates downstream pathways that promote cell survival, proliferation, and cell invasiveness. As such, IL-8 has been linked to the promotion of angiogenesis, immunosuppressive cell recruitment, and EMT transition in several cancer types. Recent studies also indicate that IL-8 can serve as a marker for COVID-19 disease progression (PMID: 22235381, 18980965, 26548401, 33584733).

Synonyms

CXCL8, CXCL8SCYB8, interleukin 8, IL8, AMCF-I, C-X-C motif chemokine 8, NAF, NAP1, NCF GCP1, GCP-1,TSG-1, K60, LAI, LECT, LUCT, T cell chemotactic factor, T-cell chemotactic factor, TCF, TSG1, Neutrophil-activating protein 1