HumanKine® recombinant human IL-7 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.1-1.4 ng/mL

Specific Activity

minimally 7.00x10⁷ IU/mg, typically 1.93x10⁸ IU/mg

Species Reactivity

human,mouse

Purity

> 95%

Publications

1

Cat no : HZ-1281-GMP

Synonyms

IL 7, IL7, IL-7, interleukin 7

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Validation Data Gallery

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  Purity of GMP-grade recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP recombinant human IL-7 (HZ-1281-GMP) stimulates dose-dependent proliferation of the 2E8 murine B lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.1-1.4 ng/mL.

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Technical Specifications

• GeneID: 3574
• Species: Human
• Expression: HEK293
• EC50: 0.1-1.4 ng/mL
• Specific Activity: minimally 7.00x10^7 IU/mg, typically 1.93x10^8 IU/mg
• Purity: > 95%
• Endotoxin: < 0.1 EU/μg
• Accession Number: P13232
• Molecular Mass: 19, 25, and 30 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-7 (IL7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration (PMID: 29663382; 29655570; 29449560)

Synonyms

IL 7, IL7, IL-7, interleukin 7

Publications

Species	Title
human	Cancer Lett; Antigen/HLA-agnostic strategies for Characterizing Tumor-responsive T cell receptors in PDAC patients via single-cell sequencing and autologous organoid application; Authors - Xu Wang