HumanKine® recombinant human IL-2 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.05-0.35 ng/mL

Specific Activity

minimally 1.70 x 10⁶ IU/mg, typically 8.63 x 10⁶ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Cat no : HZ-1015-GMP

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

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Validation Data Gallery

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  Purity of recombinant human IL-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP Recombinant human IL-2 (HZ-1015-GMP) induces dose-dependent proliferation of the CTLL2 (mouse cytotoxic T cell) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. CTLL2 cells were treated with increasing concentrations of GMP recombinant IL-2 for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.35 ng/mL.

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Technical Specifications

• GeneID: 3558
• Species: Human
• Expression: HEK293
• EC50: 0.05-0.35 ng/mL
• Specific Activity: minimally 1.70 x 10^6 IU/mg, typically 8.63 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P60568
• Molecular Mass: 17 kDa reduced,16 kDa non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

Synonyms

IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Publications

Species	Title
human	Cancer Lett; Antigen/HLA-agnostic strategies for Characterizing Tumor-responsive T cell receptors in PDAC patients via single-cell sequencing and autologous organoid application; Authors - Xu Wang