HumanKine® recombinant human HGF protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

5-25 ng/mL

Specific Activity

minimally 2.50 x 10⁵ IU/mg, typically 1.33 x 10⁶ IU/mg

Species Reactivity

human,monkey

Purity

>95%

Cat no : HZ-1084-GMP

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF

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Validation Data Gallery

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  Purity of GMP-grade recombinant human HGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP-grade recombinant human HGF (HZ-1084-GMP) stimulates dose-dependent proliferation of the 4MBr-5 Monkey epithelial cell line. Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant human HGF for 120 hours. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 5-25 ng/mL.

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Technical Specifications

• GeneID: 3082
• Species: Human
• Expression: HEK293
• EC50: 5-25 ng/mL
• Specific Activity: minimally 2.50 x 10^5 IU/mg, typically 1.33 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P14210
• Molecular Mass: 85 kDa reduced, 70 kDa non-reduced, single chain, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,monkey

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. It is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin (PMID 1530787, 2531289). HGF exerts a variety of effects on several cells, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities (PMID: 1846706; 15070743; 1383237; 1827664).

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF