HumanKine® recombinant human BMP-2 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

7.5-37.5 ng/mL

Specific Activity

minimally 7.00 x 10⁵ IU/mg, typically 7.20 x 10⁵ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Cat no : HZ-1128-GMP

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

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Validation Data Gallery

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  Purity of recombinant human BMP-2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human BMP-2 (HZ-1128-GMP) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.

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Technical Specifications

• GeneID: 650
• Species: Human
• Expression: HEK293
• EC50: 7.5-37.5 ng/mL
• Specific Activity: minimally 7.00 x 10^5 IU/mg, typically 7.20 x 10^5 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P12643
• Molecular Mass: 17 kDa reduced, 28 kDa non-reduced, homodimer, glycosylated
• Formulation: 2x PBS + 6% Ethanol, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP-2 induces bone formation and regeneration during early embryonic development (PMID 16234975). It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction (PMID 26620161). Xeno-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa.

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Publications

Species	Title
	Int J Biol Macromol; Antisense yycF and BMP-2 co-delivery gelatin methacryloyl and carboxymethyl chitosan hydrogel composite for infective bone defects regeneration; Authors - Boquan Qin