HumanKine® recombinant human beta NGF protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.5-3.0 ng/mL

Specific Activity

minimally 5.00 x 10⁵ IU/mg, typically 1.13 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1222-GMP

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF

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Validation Data Gallery

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  Purity of recombinant human beta NGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP recombinant human beta NGF (HZ-1222-GMP) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. TF-1 cells were treated with increasing concentration of recombinant beta NGF for 72 hours. Activity determination was conducted in triplicate on a validated bioassay. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.00 ng/mL.

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Technical Specifications

• GeneID: 4803
• Species: Human
• Expression: HEK293
• EC50: 0.5-3.0 ng/mL
• Specific Activity: minimally 5.00 x 10^5 IU/mg, typically 1.13 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P01138
• Molecular Mass: 13 kDa reduced, 14 kDa non-reduced, non-disulfide bonded homodimer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Beta nerve growth factor (NGF) is critical for the survival and maintenance of sympathetic and sensory neurons and may play an important role in the regulation of the immune system (PMID 16842161). The presence of beta NGF in immune cells, endocrine cells, and the CNS limbic areas suggests that beta NGF may function as an intracellular messenger to regulate the body’s response to stress (PMID 19442684). Xeno-free Recombinant Human beta NGF is expressed in human 293 cells as a non-disulfide bonded homodimeric protein with an apparent molecular mass of 13 kDa. This product is produced in a human cell expression system with serum-free, chemically defined media.

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF