HumanKine® recombinant human BDNF protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

4-40 ng/mL

Specific Activity

minimally 3.00 x10⁵ IU/mg

Species Reactivity

human

Purity

>95%

Publications

4

Reviews (1)

Cat no : HZ-1335

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor

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Validation Data Gallery

• 

  Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

• 

  Proteintech BDNF (Cat no: HZ-1335) demonstrates equivalent fold induction and a greater than 5-fold increase in potency compared to leading competitors. BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

• 

  Differentiation of SHSY5Y cells into neurons using HumanKine BDNF in the presence of Retinoic acid. The data demonstrates significantly better neuronal differentiation using HumanKine BDNF compared to leading competitor in the market.

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Technical Specifications

• GeneID: 627
• Species: Human
• Expression: HEK293
• Activity(Primary): 4-40 ng/mL
• Specific Activity: minimally 3.00 x10^5 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P23560
• Molecular Mass: 14 kDa reduced and non-reduced, homodimer, non-glycosylated
• Formulation: 10 x PBS pH 7.4 See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family, which also includes NGF, NT-3, NT-4, and NT-6. It plays a critical role in the survival, differentiation, and maturation of neurons during development. It is primarily expressed in various regions of the brain including the hippocampus, amygdala, cerebellum, and cerebral cortex. BDNF binds with the TrkB receptor, which results in the activation of downstream pathways controlling neural plasticity, neurogenesis, and stress resistance. It may also play a role in promoting angiogenesis and endothelial cell survival. Decreased levels of BDNF have been associated with several neurodegenerative diseases such as Parkinson’s, multiple sclerosis, and Huntington’s disease.

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor

Publications

Species	Title
mouse	Mol Metab; Crosstalk between the muscular estrogen receptor α and BDNF/TrkB signaling alleviates metabolic syndrome via 7,8-dihydroxyflavone in female mice.; Authors - Zhenlei Zhao
human	Hum Cell; Establishment and characterization of ZJUCHi003: an induced pluripotent stem cell line from a patient with Temple-Baraitser/Zimmermann-Laband syndrome carrying KCNH1 c.1070G > A (p.R357Q) variant; Authors - Die Chen
mouse	Adv Sci (Weinh); Integration of Functional Human Auditory Neural Circuits Based on a 3D Carbon Nanotube System; Authors - Yiyun Lou
rat	Biomed Pharmacother; Intratympanic injection of MSC-derived small extracellular vesicles protects spiral ganglion neurons from degeneration; Authors - Anning Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

paolo (Verified Customer) (02-19-2024)

worked excellent

• Applications: Cell culture
• Cell Tissue Type: SHSY5Y